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Vector Laboratories fast red counterstaining
Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
Fast Red Counterstaining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fast red counterstaining - by Bioz Stars, 2026-03
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dapi spectral counterstaining  (Akoya Biosciences)
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Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
Dapi Spectral Counterstaining, supplied by Akoya Biosciences, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi spectral counterstaining - by Bioz Stars, 2026-03
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dapi nuclear counterstain  (Vector Laboratories)
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Vector Laboratories dapi nuclear counterstain
Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
Dapi Nuclear Counterstain, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dapi counterstaining  (Vector Laboratories)
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Vector Laboratories dapi counterstaining
Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
Dapi Counterstaining, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pk 4000 hematoxylin qs counterstain vector laboratoires cat  (Vector Laboratories)
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Vector Laboratories pk 4000 hematoxylin qs counterstain vector laboratoires cat
Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
Pk 4000 Hematoxylin Qs Counterstain Vector Laboratoires Cat, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pk 4000 hematoxylin qs counterstain vector laboratoires cat - by Bioz Stars, 2026-03
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counterstaining with hematoxylin  (Vector Laboratories)
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Vector Laboratories counterstaining with hematoxylin
Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
Counterstaining With Hematoxylin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hoechst 33342 fluorescent nuclear counterstain  (Thermo Fisher)
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Thermo Fisher hoechst 33342 fluorescent nuclear counterstain
Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
Hoechst 33342 Fluorescent Nuclear Counterstain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear fast red counterstain  (Vector Laboratories)
96
Vector Laboratories nuclear fast red counterstain
Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
Nuclear Fast Red Counterstain, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear counterstaining with hematoxylin  (Proteintech)
96
Proteintech nuclear counterstaining with hematoxylin
Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.
Nuclear Counterstaining With Hematoxylin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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nuclear counterstaining with hematoxylin - by Bioz Stars, 2026-03
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Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.

Journal: Acta Physiologica (Oxford, England)

Article Title: Kisspeptin Restores Placental mTOR Signaling and Improves Glucose Homeostasis Mediators Disrupted by Maternal Hypothyroidism in Rats

doi: 10.1111/apha.70188

Figure Lengend Snippet: Assessment of placental morphology in control, hypothyroid, and Kisspeptin‐10 (Kp10)‐treated hypothyroid rats. (A) Photomicrographs of placentas at GD 14 (Hematoxylin & Eosin; Bar = 2.5 mm). (B) Absolute volume of the placental zones at GD 14 and 18. (C) Photomicrographs of PAS staining in the junctional zone (PAS; Fast Green; Bar = 50 μm). (D) Area in pixels stained by PAS at GD 14 and 18. (E) Photomicrographs of the double staining by cytokeratin + vimentin (Streptavidin‐biotin‐peroxidase; Alkaline Phosphatase‐BCIP/NBT; nuclear Fast Red; Bar = 20 μm) (F) Absolute volume of the compartments of the labyrinth zone at GD 14 and 18. (G) Surface area of the vasculature of the labyrinth area at GD 14 and 18. (H) Total fetal capillary length at GD 14 and 18. (I) Fetal capillary area at GD 14 and 18. (J) Fetal capillary diameter at GD 14 and 18. (K) Interhaemal membrane thickness at GD 14 and 18. (L) Theoretical diffusion capacity of the interhaemal membrane at GD 14 and 18. (M) Specific diffusion capacity of the interhaemal membrane at GD 14 and 18. (Mean ± SEM). Significant differences are indicated by * p < 0.05, ** p < 0.01 for one‐way ANOVA followed by SNK test, and # p < 0.05 for Student's t ‐test. BD, basal decidua; GD, gestational day; Gly, glycogen cells; Green bar, interhaemal membraneJZ, junctional zone; LZ, labyrinth zone; MVS, maternal vascular space; PAS, Periodic acid‐Schiff; SpT, spongiotrophoblast; TB, trophoblast; TGC, trophoblast giant cells.

Article Snippet: The next day, sections were kept at room temperature (2 h), and incubated with alkaline phosphatase–conjugated secondary antibody (ab6722, 1:500, Abcam, 1 h) and developed with NBT/BCIP (34 042, Thermo Scientific, 10 min) followed by Fast Red counterstaining (H‐3403, Vector Laboratories, 4 min).

Techniques: Control, Staining, Double Staining, Membrane, Diffusion-based Assay